Journal: Marine Drugs

Article Title: 4-(Phenylsulfanyl) Butan-2-One Attenuates the Inflammatory Response Induced by Amyloid-β Oligomers in Retinal Pigment Epithelium Cells

doi: 10.3390/md19010001

Figure Lengend Snippet: The effects of oAβ 1-42 and 4-PSB-2 on the viability of human adult retinal pigment epithelial cell line (ARPE-19 cells). ( A ) The expression of oAβ 1-42 in ARPE-19 cells: A11 (red), oAβ marker; DAPI (blue), nucleus; Bar, 100 µm. ( B ) A dose–response curve for RPE cells stimulated with 0.1 to 10 µM solutions of oAβ 1-42 for 48 h demonstrates the mild effect of 10 µM oAβ 1-42 on the viability of ARPE-19 cells ( n = 5–6/group). ( C ) The morphology of ARPE-19 cells after 0.1 to 10 µM of oAβ 1-42 treatment compared to control; Bar, 20 µm. The irregular shapes of cell bodies and nuclei of ARPE-19 cells (black arrows) and small vesicles in the cytoplasm (white arrows) were observed after oAβ 1-42 treatment. ( D ) Chemical structure of 4-PSB-2. ( E ) ARPE-19 cells were treated with 0.1% dimethyl sulfoxide (DMSO) in control, 4-PSB-2 (1, 25, 50, 100, and 200 µM) for 24 h. MTT analysis showed that 4-PSB-2 was not toxic to ARPE-19 cells, and 25 µM of 4-PSB-2 significantly increased cell viability.(* indicates p ≤ 0.001 between the groups).

Article Snippet: A human retinal pigment epithelial cell line (ARPE-19) was provided by Rong-Kung Tsai at Institute of Medical Sciences, Tzu Chi University (Taiwan), and cultured in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F12), containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO 2 and 95% air.

Techniques: Expressing, Marker, Control